Effects of Maternal Folate Levels and Prenatal Alcohol Exposure on Fetal Growth, Infant Outcomes and Later Development

Objectives Prenatal alcohol exposure can lead to fetal alcohol spectrum disorders (FASD), which include a broad range of cognitive, growth, behavior, and physical abnormalities. Early detection of the teratogenic effects of prenatal alcohol exposure is necessary to identify early interventions. The aim of this study is to identify the effects of prenatal alcohol exposure on growth and infant development, to determine if ultrasound imaging can be used as an early identification tool, and to determine if maternal folate supplementation can mitigate the detrimental effects on growth and infant development. Methods A prospective cohort study and randomized trial from 2008 to 2014 conducted in two sites of Western Ukraine was analyzed. A sample of pregnant women who reported moderate-to-heavy alcohol consumption during pregnancy, and a sample reporting little-to-no alcohol use during pregnancy participated in a comprehensive maternal interview and screening process. Women were further randomized into micronutrient supplementation groups. Standard ultrasound examinations during pregnancy including study specific brain growth measurements, along with blood and urine samples were obtained during follow-up visits. A biometric screening was conducted at birth, along with Bayley Scales of Infant Development-II Mental Development Index (MDI) and Psychomotor Development Index (PDI) assessments at 6 and 12 months of age. Results Estimated fetal weight (EFW), abdominal circumference (AC), biparietal diameter (BPD), transverse cerebellar diameter (TCD), occipitofrontal diameter (OFD), caval-calvarial distance (CCD), and orbital diameter (OD) were significantly reduced by alcohol exposure at third trimester ultrasound (p2(7)=18.044, p=0.012), AC at third trimester (X2(5)=17.955, p=0.003), and birth weight (X2(6)=75.058, p2(11)=63.051, p Conclusions Significant reductions in fetal growth measurements during third trimester ultrasound suggest that the effects of prenatal alcohol exposure may be detectable in late pregnancy. Significant associations between multivitamin supplementation and specific growth measurements suggest that micronutrient supplementation during pregnancy, including high doses of folate, may be an early intervention to reduce the harmful effects of prenatal alcohol exposure. Further studies are needed to identify the specific micronutrients producing these effects, and to assess the appropriate level of these micronutrients necessary to provide the greatest benefit without exceeding the safe limit

Slatted pen floors reduce Salmonella in market swine held in abattoirs

The objective of this study was to directly compare Salmonella isolation rates from pig slaughtered after 4 hours holding on slatted or solid concrete floors. Seven truckloads (replicates) of market swine ( 120 kg) from a fully integrated farrow-to-slaughter operation were studied. At unloading, groups of pigs (15-30) were sorted to 1) no-hold (20-45 minutes waiting), 2) pens with solid concrete floors (4 hours), 3) slatted concrete floors (4 hours). Postmortem samples were cultured for Salmonella. Those pigs held in pens with slatted floors (63.6%) had significantly (P \u3c 0.05) less Salmonella in their ceca than those held on solid floors (72.7%)

Peracute Infection of Swine With Salmonella

It has recently been experimentally demonstrated that pigs exposed naturally to Salmonella on the floor of abattoir holding pens can become infected between two and six hours after being placed in the pens. In addition we have demonstrated that tonsillar tissue are almost immediately culture positive following such exposure under experimental conditions. The objective of this study was to determine the shortest amount of time necessary for infection of selected tissues and to determine if the tonsil served as a route for Salmonella entry into lymphoid tissues draining the tonsil. Forty-four Salmonella-negative, market age pigs (90 to 110 kg) were fasted overnight and exposed to approximately 2 X 106 Salmonella enterica serotype Typhimurium strain X4232 (nalidixic acid resistant). The bacteria were mixed with a fecal slurry and the slurry spread on the floor of the pens. Pigs were euthanized at 15, 30, 45, 60 and 120 minutes following initial exposure. Tonsil of the soft palate, medial retropharyngeal lymph node, ileocecal lymph node, a five centimeter section of the terminal ileum, cecal contents and 100 ml of blood were cultured for Salmonella. Strain X4232 was isolated from 98 % (43/44) of tonsils. Strain X4232 was isolated from the ileocecal lymph node within 45 minutes (2/9 pigs), terminal ileum within 15 minutes (1/9 pigs), cecal contents within 15 minutes (1/9 pigs), and blood within 45 minutes (1/9 pigs). Strain X4232 was not recovered from the medial retropharyngeal lymph node, indicating that the organism did not move rapidly into this node from the tonsil of the soft palate. Results of this study indicate that Salmonella can be recovered from selected tissues in market age swine in less than the normal two hour abattoir holding time

Global Demography Expert Survey on the Drivers and Consequences of Demographic Change

Insight into the future of the world population is key for policy planning. At present, several organisations engage in developing global long-term population projections and provide varied results in terms of projected population size and structures. This diversity demonstrates that there is a need to reflect on the ingredients that feed into the projections. For this reason, the JRC, IIASA and the UN Population Division launched a survey where experts were asked to assess the validity and relevance of alternative arguments about the forces that could shape future fertility, mortality, and migration trends in the country of their choice. The results of the survey highlight that experts almost unanimously support the roles played by urbanisation, reductions in child mortality, greater educational attainment, and employment opportunities for women in decreasing fertility in high fertility countries. At the same time, life expectancy is expected to continue increasing across world regions, with the most significant improvements projected by experts in current 'low mortality' countries. Experts on migration mostly agree that climate change will lead to a rise in immigration. However, there is less agreement on the forces driving emigration. As a broad conclusion, the expert opinions seem to indicate that demographic challenges of the future do not necessarily have demographic solutions, and will instead necessitate improved adaptive capacities among all societies

Nonstationary Stochastic Resonance in a Single Neuron-Like System

Stochastic resonance holds much promise for the detection of weak signals in the presence of relatively loud noise. Following the discovery of nondynamical and of aperiodic stochastic resonance, it was recently shown that the phenomenon can manifest itself even in the presence of nonstationary signals. This was found in a composite system of differentiated trigger mechanisms mounted in parallel, which suggests that it could be realized in some elementary neural networks or nonlinear electronic circuits. Here, we find that even an individual trigger system may be able to detect weak nonstationary signals using stochastic resonance. The very simple modification to the trigger mechanism that makes this possible is reminiscent of some aspects of actual neuron physics. Stochastic resonance may thus become relevant to more types of biological or electronic systems injected with an ever broader class of realistic signals.Comment: Plain Latex, 7 figure

Noise suppression by noise

We have analyzed the interplay between an externally added noise and the intrinsic noise of systems that relax fast towards a stationary state, and found that increasing the intensity of the external noise can reduce the total noise of the system. We have established a general criterion for the appearance of this phenomenon and discussed two examples in detail.Comment: 4 pages, 4 figure